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We have ongoing studies on the detoxification of metals
by anaerobic microbial communities in subsurface aquifers. Heavy metals and
radionuclides are often found as contaminants in the subsurface. They are
present at Department of Energy sites where work on nuclear weapons and nuclear
energy took place. Our strategy is based on long-term immobilization of these
contaminants in place to reduce the risk to humans and the environment.
A variety of anaerobic bacteria including sulfate-reducing
bacteria are able to change the redox state of uranium, technetium and other
metals forming insoluble metal complexes. As this occurs dissolved metal
concentrations decrease in groundwater and the contaminants are less likely
to migrate offsite.
This project involves a collaboration between the University
of Oklahoma and Oregon State University. We employ field experimentation techniques
to look at methods to stimulate bacteria living in groundwater and sediments
to precipitate contaminants. Laboratory studies complement field investigations
and provide additional information on mechanisms of the observed processes.
Pictured above are the principle investigators
on the bioremediation project (left to right): Baohua Gu, Oak Ridge National
Laboratory; Jack Istok, Oregon State University; Jim McKinley, Pacific Northwest
National Laboratory; Jennifer Field, Oregon State Univiversity; and, Lee Krumholz, the University of Oklahoma.
Recent Publications:
Senko, J.M., T.A. Dewers and L.R. Krumholz. 2005. The effect of the form of Fe(II) and oxidation rate on microbial nitrate-dependent Fe(III) mineralogy. Appl. Env. Microbiol. 71(11):7172-7177.
Senko, J.M., Mohamed, Y., T.A. Dewers and L.R. Krumholz. 2005. A Role for Fe(III) Minerals in Nitrate-Dependent Microbial U(IV) Oxidation. Env. Sci. Technol. 39 (8); 2529-2536.
Peacock, A.D., Y-J. Chang, J. D. Istok, L. Krumholz, R. Geyer, B. Kinsall, D. Watson, K.L. Sublette and D.C. White. 2004. Utilization of Microbial Biofilms as Monitors of Bioremediation. Microbial Ecology, Volume 47, 284-292.
Wong, D., J.M. Suflita, J.P. McKinley and L.R. Krumholz. 2004. Impact of Clay Minerals on Sulfate-Reducing Activity in Aquifers. Microbial Ecology . 47:80-86.
Elias, D.A., D. Wong, L.R. Krumholz and J.M. Suflita. 2003. Characterization of microbial activities and uranium reduction in a shallow aquifer contaminated by an UMTRA disposal cell. Microbial Ecol . 46:83-91.
Senko, J.M. J. D. Istok, J.M. Suflita and L.R. Krumholz. 2002. In-situ evidence for uranium immobilization and remobilization. Env. Sci. Technol. 36:1491-1496.
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