Bioremediation of Metal Contaminated Subsurface Environments
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We have ongoing studies on the detoxification of metals by anaerobic microbial communities in subsurface aquifers. Heavy metals and radionuclides are often found as contaminants in the subsurface. They are present at Department of Energy sites where work on nuclear weapons and nuclear energy took place. Our strategy is based on long-term immobilization of these contaminants in place to reduce the risk to humans and the environment.
A variety of anaerobic bacteria including sulfate-reducing bacteria are able to change the redox state of uranium, technetium and other metals forming insoluble metal complexes. As this occurs dissolved metal concentrations decrease in groundwater and the contaminants are less likely to migrate offsite.
This project involves a collaboration between the University of Oklahoma and Oregon State University. We employ field experimentation techniques to look at methods to stimulate bacteria living in groundwater and sediments to precipitate contaminants. Laboratory studies complement field investigations and provide additional information on mechanisms of the observed processes.
Pictured above are the principle investigators on the bioremediation project (left to right): Baohua Gu, Oak Ridge National Laboratory; Jack Istok, Oregon State University; Jim McKinley, Pacific Northwest National Laboratory; Jennifer Field, Oregon State Univiversity; and, Lee Krumholz, the University of Oklahoma.
 

Recent Publications:

Senko, J.M., T.A. Dewers and L.R. Krumholz. 2005. The effect of the form of Fe(II) and oxidation rate on microbial nitrate-dependent Fe(III) mineralogy.   Appl. Env. Microbiol.   71(11):7172-7177.  

Senko, J.M., J.M. Suflita and L.R. Krumholz. 2005. Geochemical controls on microbial nitrate-dependent U(IV) oxidation. Geomicrobiol. J.   22:371-378.

Senko, J.M., Mohamed, Y., T.A. Dewers and L.R. Krumholz.   2005. A Role for Fe(III) Minerals in Nitrate-Dependent Microbial U(IV) Oxidation.   Env. Sci. Technol.   39 (8); 2529-2536.  

Istok, J.D.,   J.M. Senko, L. R. Krumholz, D. Watson, M.-A. Bogle,   A. Peacock, Y-J. Chang, and D.C. White.   2004. In Situ Bio-Reduction of Technetium and Uranium in a Nitrate-Contaminated Aquifer. Env. Sci. Technol. 38:468-475.

Peacock, A.D., Y-J. Chang, J. D. Istok, L. Krumholz, R. Geyer, B. Kinsall, D. Watson, K.L. Sublette and D.C. White. 2004. Utilization of Microbial Biofilms as Monitors of Bioremediation. Microbial Ecology, Volume 47, 284-292.

Wong, D., J.M. Suflita, J.P. McKinley and L.R. Krumholz. 2004. Impact of Clay Minerals on Sulfate-Reducing Activity in Aquifers. Microbial Ecology . 47:80-86.

Elias, D.A., D. Wong, L.R. Krumholz and J.M. Suflita. 2003. Characterization of microbial activities and uranium reduction in a shallow aquifer contaminated by an UMTRA disposal cell. Microbial Ecol . 46:83-91.

Senko, J.M. J. D. Istok, J.M. Suflita and L.R. Krumholz. 2002. In-situ evidence for uranium immobilization and remobilization. Env. Sci. Technol. 36:1491-1496.

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