Lee Krumholz, Ph.D.
Department of Botany and Microbiology
770 Van Vleet Oval
The University of Oklahoma
Norman, OK 73019

Voice: 405.325.0437

E-mail: krumholz@ou.edu

Education:

1988 Ph.D University of Illinois, Urbana-Champaign
1985  M.S.  University of Illinois, Urbana-Champaign
1982 B.Sc. Unversity of Guelph, Guelph, Ontario

 
Current Appointment:

Associate Professor
Department of Botany and Microbiology & the Institute for Energy and the Environment
The University of Oklahoma

             
Selected Publications:
creek  Zodletone Spring Microbial Observatory
Elshahed, M.S., K.N. Savage, A. Oren, M.C. Gutierrez, A. Ventosa and L.R. Krumholz . Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide and sulfur-rich spring. Int. J. Syst. Evol. Microbiol.  (In Press).
Elshahed, M.S., F.Z. Najar, B.A. Roe, T.A. Dewers and L.R. Krumholz. 2004. Survey of Archaeal diversity reveals abundance of  halophilic Archaea in a low-salt, anaerobic sulfur spring. Appl. Environ. Microbiol.  70(4): 2230-2239.
Senko, J.M., B.S. Campbell, J.R. Henriksen, M.S. Elshahed, T.A. Dewers, L.R. Krumholz. 2004. Barite deposition mediated by phototrophic sulfide oxidizing bacteria.  Geochim. Cosmochim Acta 68:773-780.
Elshahed, M.S., J.M. Senko,  T.A. Dewers, J.R. Spear, F.Z. Najar, S.M. Kenton, B.A. Roe and L.R. Krumholz .  2003. Bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring. Appl. Environ. Microbiol.  69(9):5609-5621.

bacteria  Molecular Biology of Sulfate Reducing Bacteria Project
Chang, I.S., J. L. Steger, M. M. Ramsey, J.D. Ballard and L.R. Krumholz. 2004. Differential Expression of Desulfovibrio vulgaris genes in response to Cu(II) and Hg(II) toxicity. Appl. Environ. Microbiol. 70:1847-1851.
Chang, I., J.D. Ballard and L.R. Krumholz. 2003. Evidence for Chimeric sequences during random arbitrarily primed PCR. J. Microbiol Meth. 54:427-431.
Steger, J.L., C. Vincent, J.D. Ballard and L.R. Krumholz. 2002. Desulfovibrio sp. Genes involved in the metabolism of Hydrogen and Lactate. Appl. Environ. Microbiol. 68(4):1932-1937.

jacksmall  Bioremediation of  Radionuclides by Anaerobic Bacteria Project
Elias, D.A., J.M. Suflita, M.J. McInerney and L.R. Krumholz. 2004. The periplasmic cytochrome C3 of Desulfovibrio vulgaris is directly involved in H2 mediated metal-, but not sulfate-reduction.  Appl. Environ. Microbiol.  70(1):413-420.
Istok, J.D.,  J.M. Senko, L. R. Krumholz, D. Watson, M.-A. Bogle,  A. Peacock, Y-J. Chang, and D.C. White.  2004. In Situ Bio-Reduction of Technetium and Uranium in a Nitrate-Contaminated Aquifer. Env. Sci. Technol. 38:468-475.
Peacock, A.D. Y-J. Chang, J. D. Istok, L. R. Krumholz, R. Geyer, and D.C. White. 2004. Utilization of Microbial Biofilms as Monitors of Bioremediation.  Microbial Ecology. (In Press)
Wong, D., J.M. Suflita, J.P. McKinley and L.R. Krumholz. 2004. Impact of Clay Minerals on Sulfate-Reducing Activity in Aquifers. Microbial Ecology. 47:80-86.
Elias, D.A., D. Wong, L.R. Krumholz and J.M. Suflita. 2003. Characterization of microbial activities and uranium reduction in a shallow aquifer contaminated by an UMTRA disposal cell. Microbial Ecol. 46:83-91.
Elias, D.A., J.M. Senko and L.R. Krumholz. 2003. A procedure for quantitation of total oxidized uranium for bioremediation studies. J. Microbiol Meth. 53/3:343 – 353.
Krumholz, L.R., D.A. Elias and J. M. Suflita. 2003. Immobilization of cobalt by sulfate-reducing bacteria in subsurface sediments. Geomicrobiol. J. 20(1):61-72.
Senko, J.M. J. D. Istok, J.M. Suflita and L.R. Krumholz. 2002. In-situ evidence for uranium immobilization and remobilization. Env. Sci. Technol. 36:1491-1496.

Additional Publications
Brown, C.M. and L.R. Krumholz. 2002. Integrating information literacy into the science curriculum.  College and Research Libraries. 63(2):111-123.
Krumholz, L.R., S.H. Harris and J.M. Suflita. 2002. Anaerobic microbial growth from components of Cretaceous shales.  Geomicrobiol. J. 19:593-602.
Tay, S. T.-L., H.F. Hemond, L.R. Krumholz,  C.M. Cavanaugh and M.F. Polz. 2001. Distribution and population dynamics of toluene degrading Xanthobacter autotrophicus and Mycobacterium sp. in a contaminated stream assessed by quantitative PCR. Microbial Ecol. 41:124-131.
Elias, D. A., Krumholz, L. R., Tanner, R. S. & Suflita, J. M. 1999.  Estimation of methanogen biomass via quantitation of Co-enzyme M. Appl. Environ. Microbiol. 65:5541-5545.
Krumholz, L.R., Harris, S. H., Tay, S.T. and J.M. Suflita. 1999. Characterization of two subsurface H2-utililizing bacteria, Desulfomicrobium hypogeium  sp. nov. and Acetobacterium psammolithicum sp. nov., and their ecological roles.  Appl. Environ. Microbiol.  65:2300-2306.
Tay, S. T.-L., H.F. Hemond, M.F. Polz, C.M. Cavanaugh and L.R. Krumholz. 1999. Importance of Xanthobacter autotrophicus in toluene biodegradation within a contaminated stream.  Syst. Appl. Microbiol. 22:113-118. 
Krumholz, L.R. 1998. Microbial ecosystems in the earths subsurface. ASM News. 64:197-202.
Ulrich, G.A., Krumholz, L.R. and J.M. Suflita. 1997. A rapid and simple technique for measuring sulfate reduction activity and quantifying sulfides. Appl. Environ. Microbiol. 63:1627-1630.
Krumholz, L.R., McKinley, J.P., Ulrich, G.A. and J.M. Suflita. 1997. Confined subsurface microbial communities in Cretaceous rock.  Nature 386:64-66.
Ahmann, D., A.L. Roberts, L.R. Krumholz and F.M.M. Morel. 1994. Microbe grows by reducing arsenic. Nature. 371:750.
Amann, R. I., L.R. Krumholz and D.A. Stahl. 1990. Fluorescent oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology. J. Bacteriol. 172:762-770.

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Updated 6/24/04 









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