OU—Dept. of Chemistry and Biochemistry—Stephenson Life Sciences Research Facility
Antibiotic Discovery and Resistance Group
Dr.Helen Zgurskaya Lab
Contact us: Antibioticemail@example.com
t.405-325-1678 | f.405-325-6111
09/05/2014-09/09/2014 | Interscience Conference on Antimicrobial Agents and Chemotherapy. Four posters will be presented. MORE DETAILS>
11/17/2014-11/19/2014 | International Congress on Bacteriology and Infectious Diseases. Oral Presentation. MORE DETAILS>
11/17/2014-11/21/2014 | 2014 Chemical and Biological Defense (CBD) Science & Technology Conference. MORE DETAILS>
03/29/2015-04/02/2015 | Keystone Symposium on Gram-negative Resistance. Oral presentation. MORE DETAILS>
04/25/2015-05/01/2015 | Gordon Research Seminar and Conference on Multi-drug Efflux Systems. MORE DETAILS>
ABOUT OUR GOALS
“A post-antibiotic era, in which common infections and minor
injuries can kill, far from being an apocalyptic fantasy, is instead a
very real possibility for the 21st century” - WHO 2014
Drug resistance presents an ever-increasing threat to public health
and encompasses all major microbial pathogens and antimicrobial
drugs. Some pathogens have acquired resistance to multiple
antibiotics and cause infections that are effectively untreatable.
There is a strong need for new therapeutic options, particularly
those directed against multi-resistant Gram-negative bacteria.
The major factor defining the high intrinsic and acquired multidrug
resistance of Gram-negative pathogens is their low cell wall
permeability. This permeability barrier is created by the synergistic
action of two processes that occur in different membranes of
Gram-negative cell walls. In the outer membrane, an asymmetric
lipid bilayer and narrow pores significantly reduce uptake of both
hydrophilic and hydrophobic compounds. In the inner membrane,
multidrug efflux transporters actively expel a broad range of
antibiotics from the cells.
Our research is focused on understanding fundamental principles
that govern permeation of antibiotics into bacterial cells and on
development of novel small molecule therapeutics that potentiate
activities of antibiotics.
Cell envelope of Gram-negative bacteria contains several efflux pumps varying in their composition and mechanism. Three-components pump such as AcrAB-TolC bind substrates in the periplasm and pump them out across the outer membrane (OM). Single component transporters such as MdfA pump substrates across the inner membrane (IM). These pumps act synergistically with low permeability barrier of the outer membrane (OM).